Cellulases are the group of hydrolytic enzymes such as endoglucanase (CMCase), exoglucanase, b-glucosidase (BGL) and FPase which are responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value-added products. The cellulase producing fungi were isolated from various agriculture fields. Total 21 isolates were obtained on Czapek’s Dox agar medium. Aspergillus niger was selected as most efficient enzyme producer by screening technique. Optimization of some nutritional and environmental factors like nitrogen source, temperature, pH and fermentation time were studied under submerged culture condition for cellulolytic enzyme production. Different agriculture waste material was used as carbon source. Maximum cellulolytic activity was observed in 4.2 pH media at 28°C after 96 hours in submerge condition. Wheat straw showed maximum activity of CMCase, exoglucanase, b-glucosidase and FPase were 8.38 IU/ml, 5.21 IU/ml, 0.30 IU/ml and 8.08 IU/ml, respectively followed by baggase.
Aspergillus niger, Cellulase, Lignocellulose, Wheat straw, Rice burn, Banana waste.
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