ISSN: 0973-7510

E-ISSN: 2581-690X

Hitesh Jasani1 , Nimita Umretiya1, Darshan Dharajiya1, Manthan Kapuria2, Shilpa Shah2 and Jagdish Patel3
1Department of Plant Molecular Biology and Biotechnology, C. P. College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar – 385506, India.
2Shree P. M. Patel Institute of Post Graduate Studies & Research in Science, Anand – 388001, India.
3Department of Biochemistry, PDPIAS, Charotar University of Science and Technology, Changa – 388421, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1159-1166
© The Author(s). 2016
Received: 07/02/2016 | Accepted: 03/04/2016 | Published: 30/06/2016
Abstract

Cellulases are the group of hydrolytic enzymes such as endoglucanase (CMCase), exoglucanase, b-glucosidase (BGL) and FPase which are responsible for release of sugars in the bioconversion of the cellulosic biomass into a variety of value-added products. The cellulase producing fungi were isolated from various agriculture fields. Total 21 isolates were obtained on Czapek’s Dox agar medium. Aspergillus niger was selected as most efficient enzyme producer by screening technique. Optimization of some nutritional and environmental factors like nitrogen source, temperature, pH and fermentation time were studied under submerged culture condition for cellulolytic enzyme production. Different agriculture waste material was used as carbon source. Maximum cellulolytic activity was observed in 4.2 pH media at 28°C after 96 hours in submerge condition. Wheat straw showed maximum activity of CMCase, exoglucanase, b-glucosidase and FPase were 8.38 IU/ml, 5.21 IU/ml, 0.30 IU/ml and 8.08 IU/ml, respectively followed by baggase.

Keywords

Aspergillus niger, Cellulase, Lignocellulose, Wheat straw, Rice burn, Banana waste.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.