ISSN: 0973-7510

E-ISSN: 2581-690X

Ying Xia Li1,2 , Bing Xue Dong2, Guo Xia Zheng3 and Feng Juan Zhao4
1Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, China.
2School of Life Science and Technology, Nanyang Normal University, Nanyang, China.
3School of Environment and Chemical Engineering, DaLian University, Dalian China.
4School of Biological Sciences, BingZhou University, Binzhou China.
J Pure Appl Microbiol. 2014;8(1):69-74
© The Author(s). 2014
Received: 29/07/2013 | Accepted: 08/09/2013 | Published: 28/02/2014

To screen high lipase activity marine yeast, 39 samples from seawater and sea slug were collected. The marine yeasts were isolated through adopting Rhodamine preliminary screening plate and secondarily screening with shake flask loading fermentation medium. A higher lipase activity yeast was obtained and identified as Pichia guilliermondii based on the results of routine and molecular identification. Lipase produced by P. guilliermondii was partially purified by (NH4)2SO4 precipitation and immobilized on silica. Lipolytic activities of the enzyme in free as well as immobilized crude enzyme were compared in different pH, temperature, metal ions and detergent and enzyme inhibitors. A sharp decrease in lipolytic activity was observed in case of free and immobilized crude enzyme at 50, 55 and 55 °C, respectively. The lipase activity of both free and immobilized crude extracts was inhibited when incubated with Na+, K+, Ag+, Zn2+, EDTA, PMSF, SDS and Tween80. Both the free and immobilized crude lipase forms were found to have a stimulatory effect in the presence of Mn2+, Cu2+ , Mg2+, Ca2+, Ba2+, Fe3+ and Triton X-100 showed an inhibitory effect on the activity of free as well as immobilized crude lipase.


Lipase, Lipolytic activities, Pichia guilliermondii

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.