ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

E.S. Challaraj Emmanuel* , S. Sree Priya and Sona George

Department of Life Sciences, Kristu Jayanti College (Autonomous), K. Narayanapura, Kothanur Post, Bengaluru – 560 077, Karnataka, India.
J Pure Appl Microbiol, 2019, 13 (1): 403-411 | Article Number: 5305 | © The Author(s). 2019 

Received: 04/09/2018| Accepted: 04/01/2019 |Published: 08/02/2019

Bacteria are ubiquitous in nature, survive in all environment by forming biofilm on any suitable substrata. Biofilms can harbor human infectious agents in the environment as well in the human body. E.coli is a most important species that is found in the human gastrointestinal tract and has the ability to form biofilm both In vivo and In vitro. Biosurfactants play a significant role in biotechnology for industrial and medical applications. The surface hydrophobicity is modified by the adsorption of biosurfactants, thus interferes in the microbial binding and desorption processes. The production of biosurfactants by probiotic bacteria in vivo acts as a defense weapon against most troublesome pathogenic strains in the urogenital and gastrointestinal tracts and on medical devices. The biosurfactant shows promising results than the chemical surfactant as they are better biodegradable and do not pollute the environment. Lactobacillus is a probiotic bacterium which is recognized as potential interfering bacteria by producing various antimicrobial agents. In the current study, biosurfactant producing Lactobacillus sp. isolated from homemade curd sample. The TLC result indicates that it is a lipopeptide biosurfactant. The FTIR analysis predominantly shows the presence of alkene and alkyne groups. The antimicrobial activity of biosurfactant against E.coli is compared with different concentrations of chemical surfactant SDS and the results indicate a similar antimicrobial activity. The biofilm inhibition assays suggest that the biosurfactant produced by Lactobacillus inhibits the biofilm of E. coli.


Lactobacillus, Probiotic, biosurfactant, biofilm.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.