Respiratory tract infections, especially pneumonia, are common disease in camel. The aim of this study was bacterial isolation and identification of aerobic and anaerobic bacterial agent from camels in central part of Iran. This study was conducted to 150 slaughtered pneumonic camels in the Najaf-abbad abattoir in Iran. Samples were collected aseptically from the nasal cavity, trachea, and the lungs for bacteriological examination. Standard microbiological and biochemical techniques were used for isolation and identification of bacterial genera. Out of 450 samples, 59.65% of these isolates were Gram-positive, include 55.71% aerobic and 3.94% anaerobic bacteria, and while 40.35% were Gram-negative bacteria include 35.78% aerobic and 4.48% anaerobic bacteria. The aerobic Gram-positive bacteria were Staphylococcus spp. (27.85%), Bacillus spp. (10.57%), Corynebacterium spp. (9.18%), Streptococcus spp. (8.11%) and the anaerobic Gram-positive bacteria were Clostridium spp. (3.84%) and Lactobacillus spp. (0.1%). The aerobic Gram-negative bacteria were Neisseria spp. (12.38), Pasteurella multocida (5.87%), Escherichia coli (5.37%), Pseudomonas aeruginosa (3.82%), Proteus spp. (3.73%), Klebsiella spp. (2.77%) and pasteurella haemolytica (1.92%). Anaerobic Gram-negative include Fusobacterium necrophorum (2.03%) and Bacteroides spp. (0.1%). On the basis of our study, more prevalent aerobic and anaerobic bacteria in pneumonia camels were Staphylococcus spp. and Clostridium spp. respectively.