ISSN: 0973-7510
E-ISSN: 2581-690X
The present study was conducted to isolate the yeast strain (Saccharomyces cerevisiae) from the sources such as Grapes, Molasses, Musambi, Cashew Apple, Distillary Effluent, Sorghum, Sugarcane and it is used for the study of ethanol tolerance at various concentrations from 6-14.5%. Among these the yeast strains isolated from Molasses and Cashew apple showed highest tolerance upto 12.5% and strains from Sorghum had least with 10%. Molecular characterization of yeast strain also studied using RAPD-PCR, among 63 bands produced, 6 bands were monomorphic and 57 bands were polymorphic and genetic distance of yeast strains were performed based on cluster analysis reveals that the highest genetic difference between Sugarcane and Molasses at average of 0.5 and the least genetic difference showed between Sorghum, Cashew apple and Distillary effluent at average of 0.10.
Yeast strains, Ethanol tolerance, Cluster analysis, RAPD-PCR
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