The present investigation was conducted to study the prevalence of two important Enterococcus species viz. E. faecalis and E. faecium and some of the selected phenotypic characters associated with virulence. A total of 50 fecal samples were collected from various poultry farms for isolation using a Pfizers Enterococcus agar. Preliminary identification was done by observing the colonial morphology, Grams staining and a set of 12 biochemical tests. Final confirmation was made by species-specific PCR. The biochemical characterization involved subjecting the isolates to a set of 18 different sugars to note their fermenting activity.  From 50 samples, a total of 48 isolates were identified to belong to the genus Enterococcus. The species-specific PCR showed that out of 48 Enterococcus sp. isolates, 25 (52.08%) belonged to E. faecalis, 15 (31.25%) were identified as E. faecium while rest 8 isolates were considered as miscellaneous. Biochemical characterization of isolates showed atypical reaction in fermentation of some sugars viz. Adonitol, Arabitol, Dulcitol and inulin. Virulence characterization involved attributes viz. gelatinase-production, caseinase-activity and haemolysin-production. The study showed gelatinase, caseinase and haemolysin production to a level of 84%, 72% and 40% respectively by E. faecalis while 46.66%, 40% and 53.33% were seen by E. faecium isolates respectively. Except haemolysin production all properties were more evident in E. faecalis isolates as compared to that of the E. faecium isolates.