ISSN: 0973-7510

E-ISSN: 2581-690X

A.S. Yadav1 and L.D. Kuykendall2
1Department of Genetics, CCS Haryana Agricultural University, Hisar – 125 004, India.
2Molecular Plant Pathology Lab, Plant Sciences Institute, Beltsville, Maryland – 207 05, USA.
J Pure Appl Microbiol. 2009;3(1):95-100
© The Author(s). 2009
Received: 17/08/2008 | Accepted: 22/09/2008 | Published: 30/04/2009

Spontaneous azide resistant mutants were isolated from the WT strains of Sinorhizobium fredii, Mesorhizobium ciceri and Rhizobium leguminosarum bv. trifolii and their symbiotic effectivity was tested on respective host plants either in large test tubes or in Leonard jars. A total of 12 mutants were isolated from the parent strain 54D of Sinorhizobium fredii, 11 mutants from parent strain USDA3383 of Mesorhizobium ciceri and 38 from parent strain ARC100 of Rhizobium leguminosarum bv. trifolii. The host plants inoculated with a few of the mutants of all the three species of rhizobia resistant to low doses of azide had significantly higher shoot dry weight and ARA in the nodules than the plants inoculated with the parent strains.


Azide resistance, Sinorhizobium fredii, Mesorhizobium ciceri, Rhizobium leguminosarum bv. trifolii, Symbiotic effectivity, Acetylene reducing activity (ARA)

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