ISSN: 0973-7510
E-ISSN: 2581-690X
Duck enteritis virus (DEV) is a herpes virus that cause an acute, contagious, and fatal disease. In this present article, we introduce a polymerase chain reaction (PCR) assay for DEV DNA. The method employed primer sets targeting the viral DNA polymerase gene (UL30), type I membrane protein that binds to heparin sulphate (glycoprotein C) and glycoprotein E of DEV and was able to amplify DNA fragments of the expected size from infected samples. The method will provide a valuable tool for a rapid laboratory diagnosis of DEV infection. By virtue of its high throughput format, the method may be useful for large epidemiological surveys and clarification of pathogenesis, such as latency of the virus.
Duckenteritis virus, DNA polymerase, Glycoprotein C, Glycoprotein E Polymerase Chain Reaction
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