In present studies, pathogenic bacterium, Nocardia asteroides was investigated. The growth profile of Nocardia asteroides was studied by optimizing the growth conditions by culturing 20-26 hours with maximum growth. Down-streaming process was accompanied by sonication, differential centrifugation, ultracentrifugation and detergent solubilization techniques. Partially purified membrane-bound proteins were analyzed spectrophotometricaly for membrane-bound protein contents. Reduced membrane spectra and SQR (complex II) activity showed the presence of cytochrome b while pyridine ferrohaemochrome of cytochrome c.
Cytochrome b, cytochrome c, respiratory chain, Nocardia asteroides
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