ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Martin Paul A.1 , Jayanthi D.2and Thamizhseran N.1

1St. Joseph’s College (Autonomous), Bengaluru – 560 027, India.
2Mount Carmel College, Autonomous, Bengaluru – 560 001, India.
J. Pure Appl. Microbiol., 2019, 13 (4): 2027-2033 | Article Number: 5791 | © The Author(s). 2019
Received: 17/08/2019 | Accepted: 17/10/2019 | Published: 05/12/2019

The study was to isolate, characterize and identify bacteria present in the water calyx fluid of the African tulip, Spathodea campanulata P. Beauv. Nutrient agar and nitrogen free malate (Nfb) semi-solid media were used to isolate the bacteria. Bacteria was identified using 16S rDNA sequencing. Pseudomonas putida TS 18 was found to be a new strain based on highest homology during the BLAST. Morphological and biochemical characterization of the identified bacteria was done by routine methods. This is the first study to report bacteria from the water calyx fluid of S. campanulata. Identified bacteria along with fluid content might be playing a unique role in the flower development by showing nitrogen fixing potential under in vitro conditions.


Water calyces; P. putida; 16S rDNA sequencing.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.