ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Jissin Mathew, Vani C and Jaison Titus
Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, India.
J. Pure Appl. Microbiol., 2020, 14 (1): 499-507 | Article Number: 5681 | © The Author(s). 2020
Received: 06/05/2019 | Accepted: 05/02/2020 | Published: 21/02/2020

Entomopathogenic nematodes (EPNs) were collected from soil using Galleria mellonella as bait and propagated on Wouts medium as well as new semi-solid feed developed from dog feed. The EPN was isolated from the cucumber soil from Kadayar, Thadiyoor, Kerala for the Mass production. The strains collected were identified as Steinernema siamkayai KUT1 and the symbiont bacteria identified as Xenorhabdus stockiae using advanced DNA sequencing method. Nematodes were grown on two different sets of medium viz., Wouts medium on sponge and Semi-solid dog feed in T-flasks. The Steinernema siamkayai KUT1 grown increasingly in semi-solid dog feed medium than other medium. This research suggests that EPN can be used to for the inhibition of malarial parasites in open stagnant water bodies. Breeding grounds for this species could be controlled efficiently using EPNs. Our research concludes the use of newly modified semi solid Dog feed medium containing essential nutrients can be safe and cheap enough for the mass production of EPN.


Galleria mellonella, Entomopathogenic nematode, Mass production, Dog feed semi solid media.

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.