ISSN: 0973-7510

E-ISSN: 2581-690X

Sonika Pandey , Mukesh Srivastava, Mohammad Shahid, Anuradha Singh, Shubha Trivedi, Vipul Kumar, Manoj Kumar Maurya and Yatindra Kumar Srivastava
1Biocontrol Laboratory, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur – 208 002, India.
J Pure Appl Microbiol. 2015;9(Spl. Edn. 2):553-559
© The Author(s). 2015
Received: 03/08/2015 | Accepted: 10/10/2015 | Published: 30/11/2015

Trichoderma belongs to a group of filamentous fungi, is an effective biocontrol agent. There are several antagonistic mechanisms exercised by Trichoderma species such as nutrient competition, antibiotic production and mycoparasitism. Use of biocontrol agents for the management of phytopathogens is an economical, ecological and environmental friendly process. The present study involves the isolation and characterization of volatile secondary metabolites from the culture filtrate of Trichoderma harzianum (Th. Azad). The in vitro assay of extracted volatile metabolite of Trichoderma harzianum (Th. Azad) shows growth inhibition of all the tested phytopathogens (Rhizoctonia bataticola 24%, Fusarium oxsporum ciceri 29.36%). The inhibition varied from species to species. Highest growth inhibition was achieved with Fusarium oxsporum ciceri (29.36%). Identification and profiling of volatile metabolites produced by T. harzinum (Th. Azad) yielded 15 compounds with 15 sharp peaks. Out of the different compounds obtained, 1H-Purine-6-amine (2-Fluro Phenyl) methyl), is first time found to be present in Trichoderma harzianum (Th. Azad). This compound works as a plant growth regulator.


Trichoderma, Secondary metabolites, Antagonistic, Volatiles metabolites

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© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.