ISSN: 0973-7510

E-ISSN: 2581-690X

Mukhammad Asy’ari1, I. Putu Parwata1, Pingkan Aditiawati2, Akhmaloka1 and Rukman Hertadi1
1Biochemistry Research Divisions, Bandung Institute of Technology, Jalan Ganeca no. 10 Bandung 40132, Indonesia.
2Microbial Biotechnology Research Group, Bandung Institute of Technology,  Jalan Ganeca no. 10 Bandung 40132, Indonesia.
J Pure Appl Microbiol. 2014;8(5):3387-3396
© The Author(s). 2014
Received: 14/05/2014 | Accepted: 29/06/2014 | Published: 31/10/2014
Abstract

In an effort to search new variants of extracellular lipase that stable under low water activity environment, we have been exploring the biodiversity of halophilic bacteria in a unique hypersaline environment, the Bledug Kuwu mud crater located in the mainland, Purwodadi-Grobogan, Central Java, Indonesia. Six bacteria exhibiting wide halotolerant property and high halostable lipase activity have been isolated. The 16S rRNAs genes of all six bacteria have been sequenced. Phylogenetic analysis of the 16S rRNA genes showed that five isolates belonged to moderate halophilic bacterium genus of Halomonas and Chromohalobacter, while the other one were highly similar to Pseudomonas sp., which is a low halophilic bacterium. Further analysis based on (GC-AT)% content in 16S rRNA genes from varios types of bacteria revealed general characteristics of halophilic bacteria from other types of bacteria and also highlighted the uniqueness of our isolates.

Keywords

Phylogenetic profile, Halophilic bacteria, Halotolerant level, 16S rRNA, Halostable lipase

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.