ISSN: 0973-7510
E-ISSN: 2581-690X
In an effort to search new variants of extracellular lipase that stable under low water activity environment, we have been exploring the biodiversity of halophilic bacteria in a unique hypersaline environment, the Bledug Kuwu mud crater located in the mainland, Purwodadi-Grobogan, Central Java, Indonesia. Six bacteria exhibiting wide halotolerant property and high halostable lipase activity have been isolated. The 16S rRNAs genes of all six bacteria have been sequenced. Phylogenetic analysis of the 16S rRNA genes showed that five isolates belonged to moderate halophilic bacterium genus of Halomonas and Chromohalobacter, while the other one were highly similar to Pseudomonas sp., which is a low halophilic bacterium. Further analysis based on (GC-AT)% content in 16S rRNA genes from varios types of bacteria revealed general characteristics of halophilic bacteria from other types of bacteria and also highlighted the uniqueness of our isolates.
Phylogenetic profile, Halophilic bacteria, Halotolerant level, 16S rRNA, Halostable lipase
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