Isolation and Genera Distribution of Hydrogen-oxidizing Bacteria in Loess Plateau Hippophae rhamnoides Rhizosphere

ISSN: 0973-7510

E-ISSN: 2581-690X

Isolation and Genera Distribution of Hydrogen-oxidizing Bacteria in Loess Plateau Hippophae rhamnoides Rhizosphere

Lin Wang¹, Bo Fu¹, ², Xin Guo¹, Guilin Cao¹ and Weiwei Wang¹

¹Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an, China.

²Enzyme Engineering Institute, Shaanxi Academy of Sciences, Xi’an, China.

J Pure Appl Microbiol. 2013;7(1):303-309

Received: 20/01/2013 | Accepted: 02/03/2013 | Published: 31/03/2013

Abstract

Rhizosphere of Hippophae rhamnoides was designated as research material. 39 bacterial strains were isolated by mineral salt agar (MSA) medium with a continuous H₂ supplying gas-cycling incubating system. Hydrogenase-positive strains were screened by both 2,3,5-triphenyltetrazolium chloride (TTC) and H₂ oxidizing capability tests. 6 strains with positive TTC result and strong H₂ oxidizing capability were determined to be hydrogen-oxidizing bacteria. The 6 strains were classified into genera of Bacillus sp, Aeromonas sp, Pseudomonas sp and Micrococcus sp by their cultural, morphology, physiological and biochemical characteristics. 16S rDNA sequence of strain FS2 has a homology of 99% with Bacillus sp and they are in the same branch of phylogenetic tree, indicating strain FS2 belongs to Bacillus sp.

Keywords

Hippophae rhamnoides, Symbiotic nitrogen-fixing, Hydrogen-oxidizing bacteria

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