ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anuja Mishra1,2 , Aditya Saxena1 and Surya Pratap Singh2
1Department of Biotechnology, Institute of Applied Science & Humanities, GLA University, Mathura – 281406, U.P., India.
2Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali – (Raj.), India.
J. Pure Appl. Microbiol., 2019, 13 (4): 2325-2332 | Article Number: 5915 | © The Author(s). 2019
Received: 23/10/2019 | Accepted: 09/12/2019 | Published: 24/12/2019

In the present world of industrial revolution, spills of petroleum products­­­­ and oil are one of the major sources of the contamination in the ecosystem and cause serious health hazards for humans and livestock. Therefore, the removal of these pollutants has become necessity of the time. Applications of new techniques such as bioremediation and biodegradation, over conventional methods are much more promising for safe and sustainable environment. Aim-The aim of this study is to isolate and characterize microbial strains from effluent of Mathura Refinery – Indian Oil Corporation Limited, the microscopic and biochemical studies and their potential of bioremediation. Bacteria were isolated from Mathura refinery IOCL, Based on the biochemical studies six morphologically distinct bacterial strains with promising bioremediation role were identified. To check the degradation of crude oil sample by bacterial isolates Bushnallhass media and separation funnel method were performed. Six bacteria were isolates from Mathura refinery effluentsand all six bacteria have ability to degrade the crude oil in different range. Maximum potential to degrade the crude oil were identified in Pseudomonas. This study might be important in an important step towards bioremediation techniques.


Biodegradation, Bioremediation, Petroleum products, Safe and sustainable environment.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.