The aim of this study was to investigate the in vitro antimicrobial activities of sixteen lactic acid bacteria (LAB) strains isolated from Nigerian fermented foods. The isolates showed strong activity against pathogenic bacteria, yeasts and moulds. The 16S rRNA gene sequencing and biochemical test reveal that the LAB strains belong to the genera Lactobacillus and Leuconostoc. In vitro antifungal activity was initially investigated by dual-culture plate method for yeasts and moulds, followed by agar well diffusion method using 10x concentrated cell-free supernatants (CFS) of the LAB isolates against all the target pathogens. Among these sixteen LAB isolates, Lactobacillus delbrueckii subsp. bulgaricus YML013 showed broad inhibitory spectrum against all the target pathogens, given it priority to be selected for further screening. Characterization of the selected broad-spectrum antifungal LAB isolate (L. delbrueckii subsp. bulgaricusYML013) revealed that, the antifungal activity was thermostable and the suspected compound(s) may be lipid in nature. Additionally, the assessment of the haemolytic activity and antibiotics susceptibility of the selected strain to the most commonly used antibiotics was also studied, and the results of all these screening suggestedthe LAB strain YML013 could be considered antimicrobial candidate against both microbial spoilage and enteric infections caused by pathogenic microorganisms.