ISSN: 0973-7510

E-ISSN: 2581-690X

Rashed A. Zaghloul, Hamed E. Abou-Aly, Taha A.Tewfike and Noha M. Ashry
Botany Department. (Microbiology branch), Faculty of Agriculture, Benha university, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (1): 277-290
© The Author(s). 2016
Received: 18/11/2015 | Accepted: 09/02/2016 | Published: 31/03/2016

Endophytic bacterial isolates were isolated from roots, nodules, leaves and stems of faba bean ( Vicia faba ) , pea ( Pisum sativum ) , fenugreek ( Trigonella  foenum-gracum ) , lupine ( Lupinus spp.), common bean (Phaseolus vulgaris) and rice (Oryza sativa) at flowering stage. A total of 167 endophytic isolates were screened qualitative and quantitative for cellulase and pectinase activities. Result showed that more than 55 isolates out of 167 were able to produce cellulase and pectinase enzymes. Total of 55 isolates were screened as plant growth-promoters (PGP) traits and root colonization. The highest values of log CFU was 6.4×105 of isolate TN10 inside the roots of faba bean plant. Moreover, 12 endophytic isolates produced IAA more than 25µ/ml in the presence of the precursor tryptophan. Also TN12, HN32 and RN62 isolates recorded the highest values of siderophores production. About 82% of the isolates showed positive results of HCN, 44 isolates were able to produce ammonia. The phosphate solubilization efficiency percentage were detected for endophytic isolates, 19 isolates showed the maximum range of phosphate solubilization efficiency (SE).


Root colonization, Endophytic bacteria, Celullase, Pectinase, PGP Traits, IAA, Phosphate solubilizers.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.