ISSN: 0973-7510

E-ISSN: 2581-690X

Amol Balkrushna Gulhane1, Ashish Arunrao Deshpande1, Sophia Makdoh Gogoi2 and Piruthivirajkumar Balaguru1
1Globion India Private Limited, First Floor, Plot No. 8, Devi Towers, Chandragiri Colony, Trimulgherry, Secunderabad- 500 015, India.
2Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati- 781 022, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 417-423
© The Author(s). 2016
Received: 11/11/2015 | Accepted: 17/01/2016 | Published: 31/03/2016
Abstract

The occurrence of Inclusion Body Hepatitis in commercial broilers flocks of different parts of India was studied in order to identify the Fowl Adenovirus (FadV) type responsible for the disease. The most common gross lesions observed during necropsy included hydropericardium, hepatomegaly, petechial haemorrhages on liver, yellowish discolouration of subcutaneous and abdominal fat along with swelling and paleness of kidneys. Histopathological examination revealed intranuclear basophilic inclusion bodies indicative of FadV infection. Virus isolation was attempted in-vivo, in-ovo and in-vitro in Specific Pathogen Free (SPF) birds, SPF embryonated eggs and Chicken Embryo Liver primary cells, respectively. Antibody based detection of FAdV was suitably done with the help of Agar Gel Immunodiffusion test (AGID). Polymerase Chain Reaction and Restriction Fragment Length Polymorphism analysis was performed to detect and differentiate FadV types. Sequencing and phylogenetic analysis revealed presence of FadV type 2, 4, 8 and 11 in Indian poultry.

Keywords

IBH, Fowl adeno virus, Broiler, PCR, Characterization.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.