ISSN: 0973-7510
E-ISSN: 2581-690X
The occurrence of Inclusion Body Hepatitis in commercial broilers flocks of different parts of India was studied in order to identify the Fowl Adenovirus (FadV) type responsible for the disease. The most common gross lesions observed during necropsy included hydropericardium, hepatomegaly, petechial haemorrhages on liver, yellowish discolouration of subcutaneous and abdominal fat along with swelling and paleness of kidneys. Histopathological examination revealed intranuclear basophilic inclusion bodies indicative of FadV infection. Virus isolation was attempted in-vivo, in-ovo and in-vitro in Specific Pathogen Free (SPF) birds, SPF embryonated eggs and Chicken Embryo Liver primary cells, respectively. Antibody based detection of FAdV was suitably done with the help of Agar Gel Immunodiffusion test (AGID). Polymerase Chain Reaction and Restriction Fragment Length Polymorphism analysis was performed to detect and differentiate FadV types. Sequencing and phylogenetic analysis revealed presence of FadV type 2, 4, 8 and 11 in Indian poultry.
IBH, Fowl adeno virus, Broiler, PCR, Characterization.
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