Chlorpyrifos is aorganophosphorus insecticide widely used for crop protection. Chlorpyrifos is toxic to environment, exploration of various chlorpyrifos-degrading bacteria to clean-up the pesticides is of immense importance. Six bacterial isolates were successfully isolated from chlorpyrifos contaminated soil and were named CP-1, CP-2, CP-3, CP-4, CP-5 and CP-6.  The isolates were capable of utilizing chlorpyrifos (Cp) as the sole source of carbon and were able to grow at 100 mg/L concentrations of chlorpyrifos. Effect of environmental conditions like temperature, pH and NaCl concentration was determined for all bacterial isolates.Most of bacterial isolates showed susceptibility for different antibiotics in antibiotic sensitivity test. Growth kinetics experiments showedthat the bacterial isolates were able to grow in medium containing the individual pesticide as the carbon source. Out of six bacterial isolates CP-3was identified based on16SrRNA sequence analysis.