Five variant groups were isolated from the gut of Blue gourami Trichogaster tricopterus through serial dilution, identified by using biochemical tests and bacteria were Bacillus sp., (G1) Pseudomonas sp., (G2) Enterobacter sp., (G3) Aeromonas sp., (G4) and Escherichia sp., (G5). Based on enzymatic productivity (Amylase, Cellulase, Lipase, and Protease) and antimicrobial activity Bacillus sp., Enterobacter sp., and Aeromonas sp., and mass multiplied using nutrient broth. Four different feeds such as feed I (Control), II (1ml Bacillus sp.,) III (1ml each of Bacillus sp. and Enterobacter sp.) and IV (1ml each of Bacillus sp., Enterobacter sp. and Aeromonas sp.,) were prepared. Feed utilization parameters of blue gourami Trichogaster tricopterus were studied after 21 days of rearing and all feed utilization parameters were higher in feed IV.