ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Mifthahul Jannah1, Anthoni Agustien1, Syukria Ikhsan Zam2, Rika Sari Lalfari3, Yufri Aldi4, Asiska Permata Dewi5 and Akmal Djamaan4
1Department of Biology, Faculty of Science, University of Andalas, Padang, Indonesia.
2Agrotechnology Department, Faculty of Agriculture and Animal Science, State Islamic University of Sultan Syarif Kasim Riau, Pekanbaru, Indonesia.
3Department of Chemistry, Faculty of Science, University of Andalas, Padang, Indonesia.
4Faculty of Pharmacy, University of Andalas, Padang, Indonesia.
5Departement of Pharmacy, Faculty of Medicine and Public Health, University of Abdurrab, Pekanbaru, Indonesia.
J Pure Appl Microbiol. 2018;12(3):1473-1481
https://doi.org/10.22207/JPAM.12.3.51 | © The Author(s). 2018
Received: 15/05/2018 | Accepted: 20/07/2018 | Published: 30/09/2018
Abstract

Research on the isolation and characterization of antibiotics-producing endophytic bacteria from Citrus aurantifolia leaves has been done. The isolation process was carried out by spreading spread plate isolation technique, screening of antibiotic-producing bacteria was done by disc dilution method and characterization of antibiotic-producing bacteria was done by microscopic, macroscopic and 16S rRNA analysis methods. The result of this research is four endophytic bacteria isolates that potentially produces antibiotic compounds. First, isolates with the CA01 code of fermentation fluids have inhibitory activity against Streptococcus mutans bacteria. Second, isolate with the code CA 02 able to inhibit growth of Vibrio chloreae bacteria. Third, isolates with the CA 03 code were able to inhibit bacterial growth of Salmonella thypii and E. faecalis. Fourth, isolate with CA 04 code has inhibitory activity on Salmonella thypii and Salmonella thyposa bacteria. The microscopic, macroscopic and 16S rRNA characterization for antibiotic-producing endophytic bacteria results were Bacillus cereus RNS_01 (code CA01), Pantoea agglomerans ZFJ-15 (Code CA 02), Bacillus subtilis 55C1-1 (Code CA 03)  and Bacillus pumilus SH-B11 (Code CA 04).

Keywords

Endophytic bacteria, Citrus aurantifolia, antibiotic, 16S rRNA

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© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.