Fenugreek (Trigonella foenum graecum L.) is an important seed spice, belonging to the family Fabaceae, commonly known as Methi. Wilt caused by Fusarium oxysporum is an important disease in fenugreek. Cultural, morphological and Pathogenic variability studies were conducted among 20 isolates of the pathogen and isolates were classified in to three groups. Group I consisted of five highly virulent isolates viz., Fo BAN, Fo GAN, Fo GOV, Fo SOM and Fo YAT with less number of days taken for wilting (20 to 35 days), higher virulence index of more than 2.74, growth rate of more than 18.00 mm/day, dry mycelial weight of more than 300 mg. Group II consisted of nine moderately virulent isolates viz., Fo BAI, Fo HAN, Fo HIR, Fo KAM, Fo KAV, Fo SHI, Fo SUB, Fo UPP and Fo YAD with number of days taken for wilting ranged from 35 to 50 days, virulence index of 1.99 to 2.74 and medium growth rate of growth(11.00 to 14 mm/day), medium dry mycelial weight of 275 to 300 mg. Group III consisted of six less virulent isolates viz., Fo HEB, Fo HIM, Fo MAD, Fo MADI, Fo NAR and Fo NES with more number of days taken for wilting ( more than 50 days), less virulence index of 1.24 to 1.99, slow growth rate 11 to 14 mm/day, less dry mycelial weight of 250 to 275 mg.