Pueraria tuberosa (Indian Kudzu) is an important medicinal herb used in Indian traditional medicine system since it is having array of medicinal values. The bioactive compounds from the tubers of the P. tuberosa were sequentially extracted using the range of solvents like hexane, benzene, chloroform, ethyl acetate, acetone and methanol. The phytochemical analysis revealed the presence of significant amount of total antioxidants, phenolic acids and flavonoids in the P. tuberosa tuber extract. Among the different solvent extracts, ethyl acetate and chloroform extracts possessed maximum total antioxidants, whereas methanol and acetone extracts showed maximum flavonoid and phenolic compounds. The antimicrobial activity of different solvent extracts of P. tuberosa tubers was evaluated against selected bacterial and fungal pathogens. The bacterial strains showed higher susceptible range over the fungal isolates. The findings of the present investigation revealed that the candidate plant could be further explored for possible antibiotic and antifungal agents and provides preliminary scientific validation of the traditional medicinal use of this plant.