ISSN: 0973-7510

E-ISSN: 2581-690X

R. Osoli1,5, E. Vusughi2,5, I. Feyzi3, A.R. Khlili4 and S.S. Hosseini-Asl5
1Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran.
2Azerbaijan National Academy of Scinces, Genetic Resources Institute, Baku, Azerbaijan.
3Department of Surgery, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran.
4Department of Pathology, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, Iran.
5Department of Genetics, Medical Genetics lab, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, I. R. Iran.
J. Pure Appl. Microbiol., 2016, 10 (1): 375-381
© The Author(s). 2016
Received: 08/02/2016 | Accepted: 16/03/2016 | Published: 31/03/2016
Abstract

Breast cancer is the most common cancer among the women and makes up one- third of cancers. Telomerase enzyme is a ribonucleoprotein with reverse transcriptase activity that adds TTAGGG repeated sequences to the ends of the chromosomes. The expression of the catalytic subunit of the enzyme (hTERT) in cancer cell lines and different human tumors is shown. So, in this study, the proliferation of copies of the hTERT gene in primary breast cancer was studied. Forty five Paraffin tissue samples from breast cancer patients and forty five Paraffin tissue samples from  breast non-neoplastic patients  was provided from Ardabil Imam Khomeini Hospital’s Pathology section and DNA samples were extracted with phenol-chloroform manually , then Real Time PCR was done with hTERT Forward, and Reverse primers and GAPDH Forward, and Reverse. The tumor sample was compared with the non- tumor one to investigate proliferation of copies of the hTERT gene as a prognostic indicator in patients with primary breast cancer. Real time PCR curve analysis for hTERT and GAPDH genes in tumor samples compared with non-tumor samples showed that, there is no statistical deference’s between them (p=0.322). The point of the best cut-off value was at >18.1 where sensitivity was 60 and specificity was 58. The area under the ROC curve (AUC) was 0.555. For data analysis Spss statistical software was used. Our current studies on results of amplification hTERT gene, in comprising tumors samples with non-tumors is indication of early prognosis in primary breast cancer patients. While, amplification of hTERT gene  in breast cancer patients compared to the control group indicates that there is no statistical deference’s between them and it  couldn’t be considered as prognosis factor for the primary breast cancer patients. According to conducted  research it could be suggested that the hTERT gene may be promoted through telomerase activity, but increasing the copy number in various tumor cell line is not always dependent on telomerase activity and  it likely could have an independent activity.

Keywords

Breast cancer, Telomerase, hTERT gene.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.