ISSN: 0973-7510

E-ISSN: 2581-690X

M.O. Abdel-Monem1 , T.I. El-Sayed1, K.A. El Dougdoug3, M.M. Amer1 and A.H.H. Abdel-Rrhman1,2
1Botany Department. Faculty of Science, Benha University, Egypt.
2Medical Technical Professions Dept. Higher Institute For science and technology, Mesallata, Libya.
3Microbiology Department Faculty of Agricultural, Ain Shams University, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (1): 811-821
© The Author(s). 2016
Received: 08/09/2015 | Accepted: 23/11/2015 | Published: 31/03/2016
Abstract

This study to estimate toxigenic diversity of three S. aureus strains (11SaUTI, 5SaRM and 21SaRW) isolated from clinical patient, food and water at Mesallata City, Libya. As well as to know antibiotic resistance, phage typing, biofilm formation and genoypes (icaA gene and 16s rDNA gene). Three S. aureus isolates were selected from isolates obtained from the different sources and identified by conventional biochemical tests and confirmed by VETIk kit. These isolates exhibited variation of toxigenic, antibiotics resistance, biofilm formation and phage plaque forming (phage typing). The amplified 16s rDNA gene to confirm the diversity of S. aureus isolates . In addition to molecular diagnosis to have a complete and clear image for bacterial etiology based on genetic variability. DNA genome of S. aureus isolates was used as template for PCR to amplify 16s rDNA gene. Partially, length of 16s rDNA gene could be synthesized using the internal primer sets combination in PCR to confirm its interspecific to S. aureus with an expected size 1219 bp. The replacement situation of nucleotide sequences were with percent of camera 1.067, 2.217 and 1.313 respectively. Three S. aureus isolates were recorded in gene bank under accession no. LC121093.1 (11SaUTI); LC121094.1 (5SaRM) and LC121095.1 (21SaRW).

Keywords

S.aureus; Antibiotic resistance, Biofilm, Phage typing, ica A gene and 16s rDNA genes, PCR.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.