Coffee bean contamination by aflatoxigenic fungi, ten samples were collected from local markets of the Saudi Arabia, Riyadh. Out of these samples, seven species of Aspergillus were obtained from the tested samples. Examination of coffee bean samples for the presence of toxigenic fungi was carried out. It has been found that thirteen isolates of A. flavus, twenty of A. niger , ten of A. ochraeceous, four isolates for both  A. fumigates and A. parasiticus and two isolates of A. tamari were tested for their production of Aflatoxin B (AFB) by detection under UV ligh.. Antiaflatoxigenic effect of Cardamom essential oil was studied by HPLC, where Cardamom oil at 4 % lead to the highest level on% inhibition aflatoxins B1 and aflatoxins B2 were ranged from 48.6 to 80.1% and 57.4 to 100%, respectively. As well as, natural occurrence of Aflatoxin B (µg/kg) in ten coffee samples were determining in this study and obtained that five samples were produced both aflatoxins (B1 and B2) in varying amounts. The chemical constituents of Cardamom essential oil was analyzed by gas chromatography–mass spectrometry. The major constituents were identified as 1,8-cineol (42.53%), 4- terpineol (22.6%), Beta cymene (5.22%), pinnen (5.12%), Alpha –terpinene (4.15%), Alpha terpinene (3.36%), linalool (2.68%), Gamma terpinene (1.14%), caryophyllen (1.09%),.Alpha Selinene (0.62%), Cis ocimene (0.56%), Farnesol (0.24%) and Alpha terpinol (0.17%).