ISSN: 0973-7510

E-ISSN: 2581-690X

Mausumi Debnath and Ramesh C. Srivastava
Plant Physiology and Biochemistry Research Laboratory, Department of Botany, Tripura University, (A Central University) Suryamaninanagar – 799 130, India.
J Pure Appl Microbiol. 2009;3(1):101-107
© The Author(s). 2009
Received: 12/01/2009 | Accepted: 19/02/2009| Published: 30/04/2009

The present investigation was designed to assess the effect of growth-promoting activities of Rhizobium sp_ a diazotroph, on plant growth and a number biochemical parameter including in vivo nitrogenase activity in the root rhizosphere of Zea mays L. The Rhizobium in this study was isolated from the root nodules of Vigna mungo L. and it was applied in three way, viz., seed soaking, seedling soaking, and basal treatment. Plants of Zea mays L. were raised in earthenware pots containing soil from paddy field. The results showed significant enhancement in various biochemical parameters (different fractions of leaf pigments (chlorophyll-a and chlorophyll-b, and carotenoids), total soluble sugars, phosphate buffer soluble proteins, total free amino acids, total phenol) and activities of different enzymes, viz., in vivo nitrate reductase activity and in vitro catalase activity in the leaves and in vivo nitrogenase (N2-ase) activity in the roots’ rhizosphere; and growth parameters such as lengths of root  and coleoptiles and their fresh and dry weights and leaf area also increased. The over all observations revealed that use of Rhizobium sp from the root nodules of Vigna mungo L. improved the photosynthetic efficiency, nitrogen-metabolizing capacity and plant growth to significant extent and it may be used as a source of potent bio-fertilizer for maize crop.


Soil, Rhizobium, Vigna mungo, Biomass, Maize

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© The Author(s) 2009. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.