The aim of the present study was to examine the influence of photosynthetic pigments composition in Zygophyllum, Tribulus and Fagonia plants in Saudi Arabia. We have selected Zygophyllum, Tribulus and Fagonia genera of Zygophyllacea family were collected from several geographical regions in Saudi Arabia. We have performed the pigment determination and total protein tests. It has been shown from our experiment that, leaves of studied plants were greatly varied in their photosynthetic pigments between different genera and within the same species from different location. The results showed a highly significant variations between the studied plants depending on plant species and their catchment location. The great differences of the climatic conditions between the different habitats of the studied plants resulted in the fluctuation of the estimated pigments and protein. We suppose that accumulation of protein under a certain stress suggests functional specialization for this protein and clarifies its role in the stress reaction.