ISSN: 0973-7510

E-ISSN: 2581-690X

B. Kanchanashri , M.K. Shivapraksh and C.C. Maina
1Department of Agril. Microbiology, College of Agriculture, UAS, GKVK, Bengaluru-560065, India.
J Pure Appl Microbiol. 2015;9(2):1137-1142
© The Author(s). 2015
Received: 20/03/2015 | Accepted: 20/05/2015 | Published: 30/06/2015
Abstract

Plant disease control by ecofriendly approaches are gaining importance to overcome the ill effects of chemical pesticides especially in food grains and vegetables which are consumed directly. One such approach is biopriming /microbial seed priming, which is an emerging technology with ability of beneficial microorganisms to induce resistance in plants. The present study was taken up to evaluate the impact of some strains of plant growth promoting rhizobacteria (PGPRs) viz., Rhizobium sp. (Rhi. sp) Bacillus megaterium (B. m), Pseudomonas fluorescens (P. f) and Trichoderma harzianum (T. h) individually and  in combinations for their ability to induce pathogenesis-related proteins like peroxidase (PO),   phenyl alanine ammonia lyase (PAL), polyphenol oxidase (PPO), β-1,3- glucanase and chitinase against  Fusarium sp. associated with French bean plants. In the plants that were raised using microbial (PGPR and biocontrol agents) primed seeds, an effective reduction in disease incidence was observed. These plants also developed local and systemic resistance and increased activity of PO, PAL, PPO, β-1,3- glucanase and chitinase against  Fusarium sp. was observed and several fold increase in the accumulation of defense enzyme activities was recorded. The combined inoculation treatments showed greater protection than single inoculation. On the other hand, result of microbial primed seed of French bean suggests that enhanced activities of defense enzymes may contribute to protection of French bean plants against Fusarium sp.

Keywords

Microbial seed priming, Peroxidase, Phenylalanine ammonia lyase, Phenylalanine ammonialyase, Polyphenol oxidase , β-1, 3-glucanase and Chitinases

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