ISSN: 0973-7510

E-ISSN: 2581-690X

Wu Hui , Liu Dongmei, Lu xiao-xu and Chen Hao
1The affiliated Tumor Hospital of Zhengzhou University, Zhengzhou – 450 008, Henan, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):719-725
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013

The present study was to explore the influence of up-regulated and down-regulated Cox-2 gene expression on the growth and the radiation sensitivity to transplanted tumor of esophageal cancer EC9706 in nude mice. Constructing siRNA vector for Cox-2 gene and Cox-2 gene eukaryotic expression vector, shifting to esophageal cancer EC9706 cells by the technology of lipofectamine, and obtaining stable transfected cell line by G418 screening. Taqman real-time RT-PCR and Western blot separately detect Cox-2mRNA and Cox-2 protein expression level; the experiment on transplanted tumor in nude mice detects the growth inhibiting role of up-regulated and down-regulated Cox-2 expression combined with X-radiation on esophageal cancer. The sequencing confirms the construction of siRNA vector pRNA-U6.1-siCox214 for Cox-2 gene, the results of RT-PCR and Western blot reflect that Cox-2 gene expression of transfected esophageal cancer EC9706 cells is an efficient silence. The average volume of transplanted tumor for nude mice in Cox-2 down-regulated group is significantly less than that in control group; and while the average volume in Cox-2 up-regulated group is significantly more than that in control group. Down-regulating Cox-2 expression can inhibit the growth of transplanted tumor for human esophageal cancer EC9706 in nude mice, enhance the sensitivity of tumor volume to radiotherapy, and up-regulating Cox-2 expression will make tumor radiation produce the resistance.


Esophageal Cancer, EC9706, Cox-2, Transplanted tumor, Nude mice

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© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.