ISSN: 0973-7510

E-ISSN: 2581-690X

Wu Hui , Liu Dongmei, Lu xiao-xu and Chen Hao
1The affiliated Tumor Hospital of Zhengzhou University, Zhengzhou – 450 008, Henan, China.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):719-725
© The Author(s). 2013
Received: 27/09/2013 | Accepted: 04/11/2013 | Published: 30/11/2013
Abstract

The present study was to explore the influence of up-regulated and down-regulated Cox-2 gene expression on the growth and the radiation sensitivity to transplanted tumor of esophageal cancer EC9706 in nude mice. Constructing siRNA vector for Cox-2 gene and Cox-2 gene eukaryotic expression vector, shifting to esophageal cancer EC9706 cells by the technology of lipofectamine, and obtaining stable transfected cell line by G418 screening. Taqman real-time RT-PCR and Western blot separately detect Cox-2mRNA and Cox-2 protein expression level; the experiment on transplanted tumor in nude mice detects the growth inhibiting role of up-regulated and down-regulated Cox-2 expression combined with X-radiation on esophageal cancer. The sequencing confirms the construction of siRNA vector pRNA-U6.1-siCox214 for Cox-2 gene, the results of RT-PCR and Western blot reflect that Cox-2 gene expression of transfected esophageal cancer EC9706 cells is an efficient silence. The average volume of transplanted tumor for nude mice in Cox-2 down-regulated group is significantly less than that in control group; and while the average volume in Cox-2 up-regulated group is significantly more than that in control group. Down-regulating Cox-2 expression can inhibit the growth of transplanted tumor for human esophageal cancer EC9706 in nude mice, enhance the sensitivity of tumor volume to radiotherapy, and up-regulating Cox-2 expression will make tumor radiation produce the resistance.

Keywords

Esophageal Cancer, EC9706, Cox-2, Transplanted tumor, Nude mice

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