Research Article | Open Access
Rabha EL Othmany, Hafida Zahir , Chorouk Zanane, Mostafa El louali and Hassan Latrache
Laboratory of Bioprocess and Biointerfaces, Faculty of Sciences and Technics, Sultan Moulay Slimane University, B.P. 523, 23000, Beni Mellal, Beni-Mellal 23000, Morocco.
J Pure Appl Microbiol. 2021;15(3):1705-1715 | Article Number: 6840
https://doi.org/10.22207/JPAM.15.3.67 | © The Author(s). 2021
Received: 11/01/2021 | Accepted: 25/06/2021 | Published: 01/09/2021
Abstract

Streptomyces are known for their ability to produce various secondary metabolites used in biotechnology, human medicine and agriculture. Understanding of surface properties is very interesting in the control of interfacial phenomena. The objective of this study was to investigate the effect of consistency and composition of growth medium on the physicochemical properties of the surface of Streptomyces strains. To achieve this objective, Six Streptomyces strains belonging to bioprocess and bio-interfaces laboratory are cultivated in two media Bennett (rich) and GBA (minimum). Both media are tested in solid (agar) and liquid (broth) mode. The wettability θw, electron donor character ˠ (-), electron acceptor character ˠ (+) and Surface free energy ΔGiwi are determined using contact angle measurements. On the two solid media Bennett and GBA, Streptomyces strains develop a hydrophobic surface (96.9° <θw<167.9°) with a weak electron donor character (0.3 mJm-2 < (ˠ (-)) <12.14 mJm-2) and a strong electron acceptor character (0.26 mJm-2 < ˠ (+) < 17.8 mJm-2) and a negative surface free energy ((- 11.8 mJm-2) < ΔGiwi < (-110 mJm-2)). Whereas on both Bennett and GBA liquid media, the surfaces of Streptomyces strains are generally hydrophilic (1.3° < θw < 9.33°) with a strong electron donor character (13.76 mJm-2 < ( ˠ (-)) < 70.06 mJm-2) and a positive surface free energy. By changing the composition of the culture medium, only a slight change in the degree of hydrophobicity and surface free energy of Streptomyces is observed. Regarding the effect of medium composition on the surface properties of Streptomyces, the degree of wettability and the values of surface free energy are no longer the same when the composition of the medium changes. These results could be applied in further studies interested in interfacial phenomena and microbial adhesion in biotechnological fields.

Keywords

Surface physico-chemical properties, Growth medium, agar, liquid, Streptomyces, contact angle measurement

Introduction

Streptomyces is a bacterial group belonging to the phylum Actinobacteria. They contribute to the production of many useful molecules in biotechnology. Streptomyces are characterized by a complex and special development cycle1,2. This complexity of their growth explains the limited number of studies on adhesion, surface tensions components in this important genus.

Currently, Streptomyces are used in several biotechnological processes1,3,4. However, deep studies on the surface properties and the influencing parameters are still limited5–7. The examination of the surface properties could be used for the control of the cell attachment processes in the reactors and subsequently the control of the stability8 and the metabolism of the Strain9.

Early investigations on the physico-chemistry of the microbial surface have shown the relationships between hydrophobicity and acid-base character and the implication of these parameters in the interaction of microbes with supports10. Then, the extended theory Derjaguin–Landau–Verwey–Overbeek XDLVO11 has allowed the determination of microbial surface tensions.

In very recent studies the surface properties have been used in the control and management of adhesion in different
fields12–16. It has been shown for other bacteria such as E.coli17 and Lactobacillus18 that the culture conditions affect the properties of the bacterial surface. However, for Streptomyces few reports are available in this issue5,7. To our knowledge, No studies have been performed to test the effect of the composition and consistency of culture medium on the surface properties of Streptomyces.

The objective of this research is to analyze the surface properties of Streptomyces in order to extend the knowledge about this important genus of bacteria and to provide useful data for the control of adhesion/attachment in biotechnology. We tested the effect of consistency and composition of the culture medium on the hydrophobicity, electron donor/acceptor character and surface free energy of six Streptomyces strains.

Materials and Methods

Bacterial strains, culture conditions and preparation of microbial suspension
Streptomyces strains are belonging to bioprocess and bio-interface laboratory, they are isolated from Moroccan saline soil and identified in a previous study19.

Streptomyces strains are cultivated in two Liquid mediums which were different in the composition were chosen in this study, liquid Bennett (Glucose 10g/l; yeast extract 2g/l; meat extract 1g/l; peptone 2g/l, 1 liter of distilled water) and liquid GBA (Glycerol 20g/l; soluble starch 10g/l; CaCO3 3g/l; peptone 10g/l; meat extract 5g/l). Strains were grown 7 days at 28°C under shaking. Then, cells were harvested by centrifugation for 15 min at 84000 × g, washed twice and suspended in potassium nitrate solution (KNO3, 0, 1 M).

Two agar medium used for this study agar Bennett (Glucose 10g/l; yeast extract 2g/l; meat extract 1g/l; peptone 2g/l, 1 liter of distilled water and bacteriological agar 18g/l ). Agar GBA Glycerol 20g/l; soluble starch 10g/l; CaCO3 3g/l; peptone 10g/l; meat extract 5g/l and bacteriological agar 18g/l). After 7 days at 28°C, pieces of agar containing a bacterial layer has been cut for later contact angle measurements.

Contact angle measurements and estimation of bacterial surface tension components
Briefly, bacterial cells suspended in KNO3 0.1M were filtered through nitrocellulose filter with 0.45 µm pore size using a negative pressure. The filters were left during about 30 min to air dry at room temperature until contact angle measurements. Experiments were carried out in triplicate for each strain. Contact angle measurements were accomplished using a goniometer (GBX Instruments, France) by sessile drop method. A drop of 10 μl of the liquid test (water, formamide or diiodomethane) was dispensed on the surface of the filter. Wettability was obtained directly by measuring the contact angle with water. The surface tension components are determined based on Young-Dupre equation which is related to the surfaces tension components in equation (1)

Surface free energy (ΔGiwi), Lifshitz-van der Waals components (γLW), electron donor character or Lewis base (γ) and acceptor character of electron or Lewis acid (γ+)) were estimated from the approach proposed by Van Oss20. In this approach the pure liquid (L) contact angles (θ) can be expressed as:

…(1)

As (S) and (L) mean solid phase and liquid phase respectively. The Lewis acid-base component has been obtained by:

γAB = 2√γ-γ+                                    …(2)

Cell surface hydrophobicity also called surface free energy is an expression of the Lifshitz–can der Waals γLW and acid–base γAB surface tensions21, it is usually assessed by contact angles of water, formamide and diiodomethane (purity ≥ 99%). Energies characteristics of theses solvents is presented in Table 122.

Table (1):
Energy characteristics of solvents used for contact angle measurements23: γLw : Van der Waals component of surface free energy Solvents; γ+ : Electron acceptor component of free surface energy of solvents; γ- : Electron donor component of free surface energy of solvents.

Solvent
Formule
γLw (mJ/ m2)
γab (mJ/ m2)
γ+ (mJ/ m2)
γ (mJ/ m2)
Water
H2O
21,8
51,0
25,5
25,5
Diiodomethane
CH2I2
50,8
~0
~0
~0
Formamide
CH3NO
39,0
19
2,3
39,6

The degree of hydrophobicity also called surface free energy was calculated by measuring the contact angle based on the Van Oss approach24. According to this approach the degree of hydrophobicity for a given material (i) is expressed as the interaction energy between two entities of a material (i) when it is immersed in water (w). The latter has been evaluated through surface tensions by the following equation (3):

…(3)

Contact angle measurements report the aptitude of a liquid to extent on a surface depending on its wettability. The principle of this method is to measure the angle θ of the tangent with the studied surface of the profile of a drop with defined dimensions, of a probe liquid deposited on the substrate to be analyzed.

Using water as a drop application liquid lead to conclude about surface wettability also called qualitative hydrophobicity. The use of different references liquids lead to evaluate the total surface free energy and its components based on Young-Dupre equation – presented in earlier paragraph- and also the energy components characterizing the analyzed substrate thermodynamically.

Using the approach of Van Oss20,24, it is possible to evaluate the absolute degree of hydrophobicity of surfaces. Based on the XDLVO approach, ΔGiwi (surface free energy) can be calculated. Therefore, surfaces with positive surface free energy (ΔGiwi>0) are classified as hydrophilic and surface with negative surface free energy (ΔGiwi<0) are considered as hydrophobic.

Statistical analysis
We used SPSS (IBM, version 20 for Windows) for statistical analyses and considered p<0.05 as statistically significant.

RESULTS

Physicochemical properties of Streptomyces strains in liquid GBA, agar GBA, liquid Bennett and agar Bennett media
Wettability
Cell wettability or qualitative hydrophobicity (θw), According to Vogler a surface is called hydrophobic if  (θw<65°)25 while it is hydrophilic when θw<65°25. Wettability of Streptomyces surfaces on solid and broth culture media is presented in the Fig. 2. GBA and Bennett are used in the present study. All Streptomyces grown on agar media are relatively hydrophobic (96.9°<θw<167.9°). Except Streptomyces griseorubens A23 which was hydrophilic on agar GBA (θw=17.3°). However, on liquid mediums streptomyces strains showed a hydrophilic behavior (1.3<θw<59.33). Our results showed a significant change in qualitative hydrophobicity between solid and liquid media. From GBA to Bennett media, cell surface properties exhibits slight changes. Wilcoxon text performed on the data significance 0.05.

Fig. 1. Surface wettability (θw) of Six Streptomyces strains cultivated  on  Bennett liquid, agar Bennett, GBA liquid and agar GBA media.

Fig. 2. Electron donor character (ˠ (-) in mJm-2) of Six Streptomyces strains cultivated on Bennett liquid, agar Bennett, GBA liquid and agar GBA media.

Electron donor-Electron acceptor characters
From figure 2 and figure 3 we observe that Streptomyces surface is highly electron donor (13.76 mJ m-2 ; 70.06 mJ m-2)/weakly electron acceptor (0.43 mJ m-2; 14.96 mJ m-2) when cultivated on liquid media. However, on solid media Streptomyces cell surfaces are considered as bipolar, acid components and base components are slightly similar. Electron donor values vary from 0.57 mJ m-2 to 13.75 mJ m-2 and electron acceptor vary from 0.04 mJ m-2 to 17.8 mJ m-2 (data presented in Fig. 2 and 3). Wilcoxon test confirm at 0.95 that the composition impact significantly the electron donor/ electron acceptor. Whereas, non-significant fluctuation was observed when the composition of growth medium was substituted.

Table (2):
Wettability of Six Streptomyces strains (in degree),grown in Bennett liquid, agar Bennett, GBA liquid, and agar GBA media.

WETTABILITY (°) of Streptomyces cell cultivated on GBA and Bennett medium
θw agar Bennett θw liquid Bennett θw agar GBA θw liquid GBA
Streptomyces griseorubens A3 134.54± 6.824 59.334±7.617 167.9±9.753 6.4±1.153
Streptomyces bellus A7 148±2.86 28.267±0.416 117.1334±8.295 1.3±0.45
Streptomyces bellus A10 128±6.06 15.334±5.48 136.6±6.547 3.33±1.154
Streptomyces griseorubens A14 133.1±9.12 23.434±5.11 97.7±5.183 11.433±0.28
Streptomyces griseorubens A15 143.64±3.05 28.234±5.346 96.9±0.346 8.03±0.75
Streptomyces griseorubens A23 116.57±5.79 14.234±4.186 17.3±0.5 3.0±2.64


Fig. 3. Electron acceptor character (ˠ (+) in mJm-2) of Six Streptomyces strains cultivated on Bennett liquid, agar Bennett, GBA liquid and agar GBA media.

Fig. 4. The surface free energy (SFE) (in mJm-2) of Six Streptomyces strains cultivated on Bennett liquid, agar Bennett, GBA liquid and agar GBA media.

Table (3):
Electron donor of Six Streptomyces strains (in mJ m-2),grown in Bennett liquid, agar Bennett, GBA liquid, and agar GBA media.

Electron Donar (mJ m-2) of Streptomyces cell cultivated on GBA and Bennett medium
ˠ (-) agar Bennett (mJ m-2) ˠ (-) liquid Bennett (mJ m-2) ˠ (-) agar GBA(mJ m-2) ˠ (-) liquid GBA(mJ m-2)
Streptomyces griseorubens A3 4.97±3.8 13.76±7.60 2.9±3.36 56.43±0.46
Streptomyces bellus A7 4.9±1.55 46.3±7.16 8.567±3.55 54.867±0.15
Streptomyces bellus A10 5.44±5.4 70.06±7.96 0.3±0.36 52.93±0.152
Streptomyces griseorubens A14 8.47±12.9 53.06±3.81 4.65±1.9 55.267±2.884
Streptomyces griseorubens A15 0.57±0.05 53.3±5 41.73±3.94 56.4±0.3
Streptomyces griseorubens A23 12.14±3.83 55.2±1.47 4.3±0.1 54.9±0.264

Surface free energy
Surface free energy (ΔGiwi) indicates the quantitative hydrophobicity. The surface is called hydrophobic if ΔGiwi < 0 negative and hydrophilic when ΔGiwi > 0 positive. Surface free energy of Streptomyces cells grown on Bennett and GBA media are mentioned in the Fig. 4. Bennett and GBA are used as agar and broth mode. Surface free energy of Streptomyces cultivated on agar Bennett and agar GBA are negative (from (-11.86 mJm-2) to (- 110 mJm-2)): (-11.86 mJm-2<ΔGiwi<-110 mJm-2). Streptomyces griseorubens A15 show a positive value of surface free energy (+8.86 mJm-2).

On liquid media, surface free energy values are positives and included in this interval (+12.9 mJm-2 < ΔGiwi< +33.7 mJm-2).

The average values of the surface free energy obtained after growth on Agar Bennett (-110 mJm-2<ΔGiwi< -11.86 mJm-2), Agar GBA (- 67.36 mJm-2<ΔGiwi<+8.67 mJm-2), Liquid bennet (-8 mJm-2<ΔGiwi< +33.7 mJm-2), and Liquid GBA (+23 mJm-2<ΔGiwi< +34.9 mJm-2). (See Fig. 4)

Table (4):
Electron acceptor of Six Streptomyces strains (in mJ m-2),grown in Bennett liquid, agar Bennett, GBA liquid, and agar GBA media.

Electron acceptor (mJ m-2)  of Streptomyces cell cultivated on GBA and Bennett medium
ˠ (+) agar Bennett (mJ m-2) ˠ (+) liquid Bennett(mJ m-2) ˠ (+) agar GBA (mJ m-2) ˠ (+) liquid (mJ m-2) GBA
Streptomyces griseorubens A3 1.74±0.08 14.96±1.93 3.13±0.73 0.43±0.115
Streptomyces bellus A7 5.2±0.98 10.33±4.5 1.33±0.98 1.2±0.26
Streptomyces bellus A10 6.97±1.68 5.83±1.95 0.26±0.25 5.9±0.36
Streptomyces griseorubens A14 16.4±8.05 6.23±2.34 0.55±0.07 3.2±0.6
Streptomyces griseorubens A15 0.04±0.05 3±0.78 8.6±7.44 2.2±0.1
Streptomyces griseorubens A23 1.77±1.15 6.23±2.34 17.8±0.1 2.267±0.152

Table (5):
Surface free enegy of Six Streptomyces strains (in mJ m-2),grown in Bennett liquid, agar Bennett, GBA liquid, and agar GBA media.

  Surface free energy of Streptomyces cell cultivated on GBA and Bennett medium
ΔGiwi (mJ m–2) agar Bennett ΔGiwi (mJ m–2) liquid Bennett ΔGiwi (mJ m–2) agar GBA ΔGiwi (mJ m–2) liquid GBA
Streptomyces griseorubens A3 (-) 60.3±3.8 (-) 8±3.40 (-) 56.933±11.73 34.9±0.6
Streptomyces bellus A7 (-) 56.17±1.11 12.9±8.62 (-) 47.833±2.74 31.3±0.5
Streptomyces bellus A10 (-) 38.98±5.4 33.7±11.08 (-) 93.033±2.02 23.0±0.7
Streptomyces griseorubens A14 (-) 11.86±12.9 23.1±7.37 (-) 67.367±9.58 29.4±1.7
Streptomyces griseorubens A15 (-) 110±0.05 29.8±5.05 8.67±7.90 32.5±0.5
Streptomyces griseorubens A23 (-) 41.61±5.02 24.3±5.64 (-) 15.567±7.13 30.2±0.5
DISCUSSION

Streptomyces cells modify their wettability behavior
The measurement of the surface wettability of Streptomyces strains has shown that the surfaces of these strains are generally hydrophobic on solid medium (agar) and hydrophilic on liquid medium.

The development cycle of Streptomyces is characterized by its complexity. Spores undergo germination then vegetative hyphae develop into aerial hyphae. Aerial hyphae and spores are described in the work of Claessen and Elliot by specific proteins on the surface which allows the cells to rise from the liquid medium. We have characterized the surface after 7 days of incubation26,27.

Moreover, certain types of proteins expressed on the aerial hyphae called Chaplin. The latter are characterized by their amphipatic nature and anchored in the cell wall. These proteins undergo an assembly in such a way that the hydrophobic part is oriented towards the outside of the cell thus conferring hydrophobicity to the aerial mycelium which contains them. Chaplins can be used to modify a variety of hydrophilic and hydrophobic surfaces in vitro thereby changing their nature. Assembly on glass leads to a protein coating that makes the surface hydrophobic28. On liquid medium the expression of surface proteins is neither as a solid medium where the cells undergo a transition from the vegetative phase to the aerial phase29,30.

In addition, it was demonstrated that the availability of glucides and yeast extract in the fermentation medium impact the surface hydrophobicity of Lactobacillus acidophilus. Cell wall hydrophobicity, which is low for cultures grown in complete medium and in the absence of carbohydrates, becomes quite high for cultures grown in medium without peptones and medium without yeast extract18.

Kim et al. explained that Streptomyces growth in liquid media is characterized by the expression some specific molecules on the surface and the existence of DNA extracellular which make these pellets hydrophilic31.

Electron donor / electron acceptor of Streptomyces surfaces
The consistency of the culture medium affects significantly the electron donor/acceptor character, whereas the effect of the composition is not very pronounced. This change in the electron donor/acceptor character is probably due to the surface proteins. Various works concerning this issue are carried out on other bacteria. In fact, Briandet showed that cell surface charge, hydrophobicity and electron donor/electron acceptor character of Listeria monocytonenes ScottA strains change when glucose or lactic acid are added to growth medium23. In fact proteins structures are influenced by the amino-acid source available in the medium. In the same manner, other scientists showed the impact of growth conditions on physicochemical properties of bacteria like Enterococcus faecalis21, Lactobacilli strains32, Enterocci strains33.

Surface free energy (SFE) of Streptomyces variations
Surface free energy (SEF) is from the important parameters required in interfacial phenomena. The obtained results from contact angle measurements and calculations of surface free energy of the surface of Streptomyces grown on broth Bennett, agar Bennett, liquid GBA and agar GBA showed a significant impact of the consistency and composition of surface free energy. These results lead to suggest that Streptomyces may use their advanced quorum sensing in detecting the environmental conditions. In fact, Jones et al. have highlighted that Streptomyces could exist as an exploratory growth. This bacterial group could detect organic volatil compounds coming from fungi and changes the morphology and the cells organization34.

Many interesting studies are carried on other bacteria in order to understand the factors affecting the Physico-chemistry of the bacterial surface. Latrache et al. demonstrated that Surface free energy and other surface tensions of Escherichia coli are not equivalent in solid LB medium as in liquid LB medium17. Also the environmental conditions such as pH, ionic strength or presence of antimicrobial substances35 affect surface physicochemical properties. The surface tensions of Staphylococcus aureus and Escherichia coli are influenced by pH and ionic strength, their influence seemed to be significant on experimental adhesion assessed by scanning electron microscopy36. A deep analysis of the molecules of cell wall leads to explain the relationship between chemical composition of the cell wall and the electron donor/electron acceptor. Phosphate groups have a key role in evaluating the property of the electron donor (base). Amine groups decrease electron acceptor property (acid)37. Further Electron donor character electron acceptor character of bacterial surface are related to its composition on functional groups38. Contact angle measurements, xylene adsorption and cell partition in a polyethylene glycol/dextran (PEG/DEX) biphasic system are used to determine cell surface hydrophobicity of Escherichia coli. Chemical composition of bacterial surface obtained by XPS lead to determine C/O and C/N ratios also it can provide molecular content of the bacterial surface. A correlation obtained between these two parameters lead to conclude physicochemical properties from chemical analysis of cell surface39.

The conclusions and rules established derived from other bacteria could not be valuable for Streptomyces, because they have specific amino-acids in their cell wall40,41. However, Del sol reported that characterization of cell surface of Streptomyces using atomic force microscopy (AFM) indicated that vegetative hyphae are hydrophilic while aerial hyphae are hydrophobic. Vegetative hyphae have a relatively smooth surface and are attached to an inert silica surface by means of a secreted extracellular matrix. Aerial hyphae, which appear in aerial growth are poorly decorated with fibers42. In addition, analysis of extracted and identified extracellular proteins from vegetative and aerial hyphae showed different expression of proteome43.

From analysis of the cited literature above, The difference in surface free energy could be explained by the variation of the expression of proteins in each medium, since it is known that the culture medium in which bacterial survive plays a key role in cellular metabolism, as well as in the control of gene expression. Streptomyces wall depends on the composition of the medium and the availability of nutrients.

CONCLUSION

Physicochemical properties of cell surface Streptomyces changes significantly depending on the consistency and composition of culture medium on which Streptomyces were grown. In fact, on both agar media the surfaces of Streptomyces were qualitatively and quantitatively hydrophobic, weak electron acceptor, weak electron donor (bipolar surface). However, on the two liquid mediums, the surfaces showed a qualitatively and quantitatively hydrophilic character, high electron donor character, weak electron acceptor character (monopolar surface). In addition, the levels of the qualitative hydrophobicity, the electron donor / electron acceptor characters and the quantitative hydrophobicity (surface free energy) differ according to the composition of growth medium. Thus, the characterization of surfaces properties of Streptomyces could be a key factor in the control of interfacial phenomenon.

Declarations

ACKNOWLEDGMENTS
None.

CONFLICT OF INTEREST
The authors declare that there is no conflict of interest.

AUTHORS’ CONTRIBUTION
LH, HZ, ME conceived and designed the experiments; REO and CZ performed the experiments and analyzed the data; REO and HZ wrote the paper. All authors read and approved the manuscript.

FUNDING
None.

ETHICS STATEMENT
This article does not contain any studies with human participants or animals performed by any of the authors.

AVAILABILITY OF DATA
All datasets generated or analyzed during this study are included in the manuscript.

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