ISSN: 0973-7510

E-ISSN: 2581-690X

N.D. Punit Kumar1 , C. Karegowda1, K.B. Rudrappa3, M. Mahesh2, T.G. Manu1, R. Murali1, H. Nagaraj1, B. Mahantesh1 and T.H. Kavita1
1Department of Plant Pathology, College of Agriculture, University of Agricultural and Horticultural Sciences, Shivamogga-577 204, Karnataka, India.
2Department of Plant Pathology, College of Agriculture, Bimarayanagudi, University of Agricultural Sciences, Raichuru, Karnataka, India.
3Department of Plant Pathology, College of Agriculture, Banglore, University of Agricultural Sciences, Banglore, Karnataka, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1603-1606
© The Author(s). 2016
Received: 03/02/2016 | Accepted: 09/03/2016 | Published: 30/06/2016

The paper gives an account of the variations in nutritional and physiological characteristics found in different isolate of Cercospora nicotianae causing frog eye leaf spot disease of tobacco, in India. The pathogen under study varied in their ability to grow in different temperature and pH levels and to utilize different carbon and nitrogen sources. Fructose was found to be the best source of carbon for the growth and sporulation of this pathogen. Among the nitrogen sources tested, Ammonium nitrate supported the maximum growth of the fungus followed by potassium nitrate.The optimum temperature range is between 20 to 30ºC levels are identified as ideal for growth and sporulation. The least dry mycelial weight was found at below 200C  and above 400 C.  The pathogen grew well at pH of 5 to pH 6 favoured maximum growth and development of the fungus. Whereas, least growth was observed at pH 3.


Tobacco, Frog eye leaf spot, Cercospora nicotianae, Nutritional, Physiological studies.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.