Staphylococcus aureus a leading pathogen of all nosocomial infections has acquired resistance to all the major antibiotics. These antibiotic resistant markers are usually present in different transposons whose transposition in the bacterial chromosome results in over expression of resistance marker genes conferring resistance to bacteria. In the present study S.aureus ATCC 12600 which is highly sensitive to aminoglycosidic antibiotics was exposed to UV rays for 10 sec or treated with 1ng of Ethidium bromide resulting in over expression of Spc1 enzyme thus making this pathogen resistant to streptomycin. The kinetics of Spc1 in both induced and uninduced S.aureus showed Vmax, Km and Kcat; 0.718±0.02µM of Adenylation/mg/min, 0.026±0.005µM, 3.37±0.2/min and 0.015±0.01µM of Adenylation/mg/min, 1.22±0.02 µM and 0.07±0.01/min respectively. The Spc1 gene part of Tn5405 transposon was found to be located on chromosome of S.aureus and on inducing with UV or Ethidium bromide this transposon probably transposed to a region on the chromosome where the expression of Spc1 increased markedly in the pathogen conferring streptomycin resistance to the S.aureus ATCC 12600.