ISSN: 0973-7510

E-ISSN: 2581-690X

Hemant Kumar Khuntia1, Surya Kanta Samal1, Sudeep Ranjan Nayak2, Ashok Kumar Sarangi3, Priyabrata Mohanty4, Santanu Kumar Kar1 and Bibhuti Bhusan Pal1
1Department of Microbiology, Regional Medical Research Centre, Bhubaneswar – 751 023, India.
2Department of Microbiology, Allahabad Agriculture Institute (Deemed University), Allahabad, India.
3School of Biotech Sciences, TACT, Bhubaneswar – 751 024, India.
4Department of Botany, Utkal University, Bhubaneswar, India.
J Pure Appl Microbiol. 2008;2(1):197-204
© The Author(s). 2008
Received: 11/02/2008 | Accepted: 26/03/2008 | Published: 30/04/2008
Abstract

This study was undertaken to analyze the cholera situation during July to November 2000 six month after the super-cyclone in saline tract of Orissa, India. Hundred ninety eight rectal swabs collected from hospitalized diarrhoea cases were subjected for bacteriological analysis. Of the 162 culture positive cases, V.cholerae 81 (50%), EPEC 1 (0.6 %), EHEC 3 (1.8%), EAggEC 2 (1.2%), Shigella flexneri 3 (1.8%) and Salmonella spp. 4 (2.5%) were isolated. Quadriplex and monoplex PCR assay revealed that, 51(31.5%) were V. cholerae O1 and 30 (18.5%) V. cholerae O139; carried ctxA, tcpA (Biotype El Tor), zot, ace and toxR except 3
V. cholerae
O139 negative for ctxA gene. Incidence of V. cholerae six month after the super cyclone was found significantly higher than the pre-cyclonic period (P < 0.5). Strains of
V. cholerae
O1 was observed to be resistant to nalidixic acid, furazolidone, streptomycin, co-trimoxazole, ampicillin & neomycin. Except for co–trimoxazole, the resistant pattern of O139 was identical with that of O1 strains.  The study revealed that V.cholerae O1 & O139 were the potential organism for cholera outbreaks in coastal Orissa, where ctxA & tcpA genes played a major role for pathogenesis. Incidence and emergence of fluroquinolone resistant V. cholerae O1 and O139 and nalidixic acid resistant O139 sero group should be closely monitored.

Keywords

Vibrio cholerae, Quadriplex PCR, Diarrhoea, Antibiotic, Resistance

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© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.