Diabetes mellitus is a chronic disorder and affects large segment of population and is a major public health problem. The infection leads to the early development of complication even after a trivial trauma, the disease progresses and becomes refractory to antibacterial therapy. Early diagnosis of microbial infections and screening for mechanism of drug resistance is aimed to institute the appropriate antibacterial therapy and to avoid further complications. Beta lactamases are enzymes responsible for the resistance to beta lactam antibiotics. This study is aimed at the detection of various types of beta lactamases present among the gram negative bacilli isolated from diabetic foot infection. Adult diabetic patients admitted for lower extremity infections from July 2008 to Jan 2010 to the medical wards and intensive care unit of medical teaching hospitals were included in the study.  179 gram negative bacilli were isolated and screened for the presence of extended spectrum beta lactamase, AmpC lactamase, Metallo beta lactamase and confirmed by the respective confirmatory tests. 54.7% produced extended spectrum beta lactamases, 10.1% AmpC beta lactamase and 71.7% strains produced metallo beta lactamases. b-lactamase producers are emerging threat and cause of concern for the clinicians, as it results in the resistance to penicillin, cephalosporins and limits therapeutic options. Screening techniques should be performed routinely to detect these b-lactamase producers so that suitable antimicrobial therapy can be instituted