ISSN: 0973-7510
E-ISSN: 2581-690X
Hospital based study was established in Odisha, India to understand the epidemiology of diarrhoeal disease among children. During the year 2006 samples were collected from hospitalized children less than 14 year of age who had diarrhea. Colonies from each culture with morphology typical of that of Escherichia coli were tested for the presence of elt gene encoding heat labile enterotoxin by PCR assay. Of the presumptive E.coli isolated from 151 children from a total of 250 diarrhoeal children, 7% (11/151) were found entero toxigenic E.coli (ETEC) carrying elt gene. The antibiotic susceptibility pattern of ETEC was ciprofloxacin, norfloxacin, chloramphenicol, gentamycin and tetracycline. RAPD DNA finger printing analysis revealed genetic homogeneity among our ETEC strains. ETEC is one of important enteric pathogens causing diarrhea among children in Odisha which should be closely monitored.
Diarrhea, E. coli, ETEC, elt, Antibiotics, RAPD and Clonality
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