The present study was undertaken with following aim and objectives. Isolation and identification of different aetiological agents causing dermatophytosis and to study antifungal susceptibility testing of isolated fungi. Isolation and identification was done by macroscopic, microscopic and biochemical tests.In-vitro drug susceptibility testing was done by broth macro dilution method The present study for isolation, identification and in-vitro drug susceptibility testing was done on 250 clinically diagnosed cases of dermatophytosis.  Out of 250 cases of dermatophytosis, 138 cases (55.2%) were positive in direct microscopic examination (KOH) and total of 106 cases (42.4%) were positive in culture. 102cases (40.80%) were positive in direct examination (KOH).MIC range of ketoconazole for all the isolates was 0.25-8µg/ml.MIC range for all the isolates against fluconazole was 0.25-16µg/ml.MIC range for all  the isolates against tolnaftate was 0.5->32µg/ml. This study highlighted that,in vitro susceptibility pattern indicates that dermatophytes are more susceptible to ketoconozole and fluconazole than tolnaftate.