Antiseptics are widely used in fish diseases to control the growth of fungal on living tissues (External body surface and Gills). The current study included the antifungal effects of antiseptics (Malachite green 5mg/l, Methylene blue 10mg /l, Potassium permanganate 20 ppm, NaCl 5% , Quaternary ammonium 0.5 ml/l, Copper sulfate 0. 5 mg/l,  Formalin 250 ppm and Povidone iodine 100mg/l) against Branchiomyces sanguinis, where several laboratory tests were used to evaluated the antifungal activity of antiseptics that used in the this study include Minimum inhibitory concentration, Spore growth test, Radial growth assay, Agar-well diffusion method of sensitive test. The results showed that Malachite green, Copper sulfate , Quaternary ammonium and Formalin were highly effectiveness against Branchiomyces sanguinis, while the Methylene blue, Potassium permanganate, NaCl and Povidone iodine were least effective in comparison with other antiseptics used in this study.