ISSN: 0973-7510

E-ISSN: 2581-690X

Ehab M.R. Metwali1,2,
Hemaid I.A. Soliman3, S.M. Howladar4, H.S. Al-Zahrani1 and
M.P. Fuller5

1Biological Science Department, Faculty of Science,King Abdulaziz University,21589 Jeddah, Saudi Arabia.
2Botany Department, Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt.
3Plant Genetic Recourses Department, Desert Research Center,El-Matariya 11753, Cairo, Egypt.
4Biology Department, Faculty of Science, Albaha University, Albaha, Saudi Arabia.
5School of Biological Sciences, Faculty of Science and Environment, Plymouth University, Plymouth, PL4 8AA, UK.
J. Pure Appl. Microbiol. 2014, 8(6):4373-4384
© The Author(s). 2014
Received: 18/10/2014 | Accepted: 03/12/2014 | Published: 31/12/2014
Abstract

A preliminary study was conduct to determine the effect of  iso-osomatic water deficit on three fig (Ficus carica L.) cultivars using mannitol as a casual agent for drought stress.   The shoots of Fig  cultivars (i.e., Black Mission, Brown Turkey and Brunswick) were sub-cultured on MS medium supplemented with 3 mg L-1 BAP and 0.5 mg L-1 2iP  at different concentrations of mannitol (0.0, 50, 100, 150, 200, 250, and 300 mM L-1) for 35 days under in vitro culture conditions. Number of newly formed shoots, shoot length, leaves number per shoot,  necrosis %, fresh weight, dry weight, chlorophyll content, relative water content were determined at regular intervals. The Obtained results showed that all growth parameters were reduced by increasing the mannitol concentration except for the percentage of necrosis %  which was increased with a significant difference among all treatments. Black Mission was found to resist Mannitol up to concentration of 300 mM L-1, while Brown Turkey and Brunswick cultivars was found to resist mannitol up to the concentration of 250 mM L-1only , where both of this cultivars was not survived under 300 mM L-1. Genomic DNA of the  Ficus carica L cvs. Black Mission, Brown Turkey and Brunswick were extracted and were used in performing Randomly Amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Three arbitrary oligonucleotide primers showed that the number of amplified fragments different from one cultivars to another and this  indicating that the fig cultivars are not always identical in their DNA ability to be amplified, which may due to unsimilarity in their genetic structure and common selection history. The use of RAPD molecular markers will be more effectiveness than ISSR to distinguish between the different fig cultivars.

Keywords

Fig (Ficus carica L.), Drought, Mannitol, Proline, Chlorophyll, Polymorphism, Molecular markers

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.