Several scolocidal agents have been used to eradicate the infective protoscoleces, however recurrences occur. The objective of this study was determining of the scolicidal effects of 3% Hydrogen peroxide, 0.5% Silver nitrate and 20% hypertonic saline on protoscoleces of hydatid cyst. Protoscoleces were collected aseptically from sheep and cattle livers containing hydatid cyst. Hypertonic saline was used as positive control, while normal saline was used as negative control. These scolocidal solutions were used for 5,10,15 and 20 minutes in the experiments. Viability of protoscoleces was assessed by 0.1% eosin staining . Normal saline had no significant protoscolicidal effect. However, 20% Hypertonic saline and 0.5%Silver nitrate solution revealed higher protoscolicidal effect than 3% Hydrogen proxide. The results showed that hypertonic saline 20% and  0.5% Silver nitrate solution is highly effective in killing protoscoleces of Echinococcus granulosus in vitro.