The antimicrobial principle from the culture filtrate of Streptomyces sp. TC1 against bacterial leaf blight pathogen Xanthomonas oryzae pv. oryzae (Xoo) was isolated by bioassay guided separation technique. The structure of the isolated compound has been identified as 6-hydroxy-7-methoxycoumarin using extensive spectroscopic studies. The isolated compound evaluated for its biological properties including antimicrobial, antioxidant, and protein binding activities. The compound produced an inhibition zone of 13-19 mm against various isolates of bacterial leaf blight pathogen in agar well diffusion method. The isolated compound possessed moderate antioxidant activity in the tested antioxidant assays. The docking studies of compound were carried out with specific proteins of interest which responsible for bacterial leaf blight disease in rice. Based on the binding energy and number of hydrogen bonds formed in protein docking studies, it is inferred that isolated compound exhibit antimicrobial activity against receptor protein 3S8M and 3H2G. These results indicate that the Streptomyces sp. TC1 can be utilized as a biocontrol organism for the management of bacterial leaf blight disease in rice.
Streptomyces, Antimicrobial, Antioxidant, Coumarin, Docking
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