Our results in the present study demonstrated that bacteria isolated from rhizosphere of plants had potential inhibitory activity against major fungal plant pathogens. Totally 116 bacterial isolates have been obtained from the rhizospheres of various herbal plants at various locations of Qinghai province in China. Some of the isolates have strongly inhibited the growth of the major plant pathogenic fungi, while, certain bacteria have not shown any antagonistic activity. The screening results indicated that tested bacterial species exhibited varying degree of antagonistic potential against most of the plant pathogenic fungi, and these strains can be used as promising biocontrol agents. Studies are under the way to find the possible action mechanisms and to seek the application of effective bacterial isolates in greenhouse conditions for biocontrol of various diseases in the vegetable crop plants.