Protoplasts of A. terreus were transformed by pTRLI plasmid containing lovE gene as regulator gene in lovastatin biosynthesis and ptrA gene as pyritiamine resistance gene. Transformats were selected for ability to grow on Czapex-dox agar containing pyrithiamine. Transformats were stable and grew on Czapex-dox agar containing pyrithiamine for 5 generations. A. terreus transformant is tested for lovastatin productivity using medium fermentation. This research showed that copy number of lovE gene in A. terreus increased significantly lovastatin productivity. The lovastatin productivity of A. terreus recombinant was 693 ppm, which is 93% hingher than the parental strain (398 ppm) at 28oC for 7 days and 200 rpm.
Lovastatin, Aspergillus terreus recombinant, Transformation, pTRLI plasmid
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