ISSN: 0973-7510

E-ISSN: 2581-690X

Vishal Mahajan1 , Harmanjit Singh Banga2 and Gursimran Filia1
1Department of Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141 004, India.
2Depatment of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141 004, India.
J Pure Appl Microbiol. 2014;8(1):753-756
© The Author(s). 2014
Received: 28/03/2013 | Accepted: 03/06/2013 | Published: 28/02/2014

Rapid and precise diagnosis in natural field cases of bovine abortion warrants use of most sensitive and reliable diagnostic methods. In the present study, histopathological, immuno-histochemical and PCR technique(s) have been applied for diagnosis of abortion cases due to Salmonellosis. Histopathologically, multifocal lymphoid granulomas were observed in liver of aborted fetuse(s) which showed positive immuno-reactivity to the anti-Salmonella polyclonal antibody. PCR for diagnosis of salmonellosis in the aborted bovine fetuses found two positive samples out of the total 113 samples tested. Primers S18/ S19 pair amplified a 159 bp region of the sequence encoding an ompC gene of Salmonella species.


Abortion, Immuno-histochemistry, PCR, Salmonellosis

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© The Author(s) 2014. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.