Illicium verum, which has been planted more than 3.3×105 ha, is the expensive medicinal plant in south China. However, rich extractives in Illicium verum biomass were severely wasted for the inefficient extraction and separation processes. In order to further utilize the biomedical resources, the four extractives were obtained by JYBS extraction, and the immunology moleculars of JYBS extractives were investigated and analyzed by GC-MS. The result showed that the first-stage extractives contained 89 components including anethole(54.24%), 2-hydroxy-2-(4-methoxy-phenyl)-n-methyl-acetamide(4.81%), etc; the second-stage extractives had 25 components including anethole(85.17%), d-limonene(1.90%), etc; the third-stage extractives contained 81 components including cyclohexyl-benzene(16.58%), 1-ethyl-1,2,3,4-tetrahydro-naphthalene(10.47%), etc; and the fourth-stage extractives contained 98 components including undecane(7.58%), decane(6.48%), etc. And The JYBS extractives of Illicium verum biomass had a main retention time between 10-20 min. what’s more, the JYBS extractives contained many biomedical and immunology moleculars, such as anethole, stigmasta-4,6,22-trien-3b-ol,g-sitosterol,squalene, and so on. So the functional analytical results suggested that the JYBS extractives of Illicium verum had huge potential in immune and other biomedicines.