Immune Responses of Conserved 32 KD fragment from N-terminal of H. pylori cagA gene

ISSN: 0973-7510

E-ISSN: 2581-690X

Immune Responses of Conserved 32 KD fragment from N-terminal of H. pylori cagA gene

D. Esmaeilli¹, A. Mohabati Mobarez¹, A.H. Salmanian², A. Zavaran Hosseini³ and R. Hosseini Doust⁴

¹Department of Bacteriology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.

²National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

³Department of Immunology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.

⁴Department of Microbiology, Faculty of Medical Science, Baqyatollah University, Tehran, Iran.

J Pure Appl Microbiol. 2010;4(1):117-123

Received: 21/10/2009 | Accepted: 13/12/2009 | Published: 30/04/2010

Abstract

H. pylori is gram-negative, microaerophilic and motile bacterium that has been associated with gastritis, peptic ulcer and adenocarcinoma. Aim of this research was study of conserved rCagA 32 KD fragment immunogenicity characterizations from amino end of cagA gene. CagA gene was amplified by PCR and target 841 bp fragment was cloned into proper vectors and was approved with enzymatic digestion and PCR.

We showed that 32 KDa our designed recombinant CagA proteins from amino ends of H. pylori was immunogenic and enable to induce proper immune Th1 responses in mice. The results showed that designed rCagA had the same antigenicity as native CagA and also unlike whole cagA is completely conserved.

We recommended this conserved and immunogenic fragment of CagA for developing immune-detecting method for CagA+HP and proper candidate for formulation of multi-component vaccine.

Keywords

Helicobacter pylori, Cloning, 32 KD rcagA

Download PDF

Article Metrics

Article View: 682

JPAM.4.1.17 (216 downloads)

Share This Article

© The Author(s) 2010. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.