ISSN: 0973-7510

E-ISSN: 2581-690X

Neda Razavi Davoodi1, Seyed Davar Siadat1 , Farzam Vaziri1, Jalil Vand Yousefi2, Naser Harzandi2, Ali Rafi2, Bahareh Rajaei1, Mehrangiz Zanganeh3, Alireza Japoni Nejad1, Shahin Najar Peerayeh4 and Ahmad Reza Bahrmand1
1Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
2Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
3Islamic Azad University Tehran Medical Branch, Tehran, Iran.
4Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Pure Appl Microbiol. 2015;9(1):467-471
© The Author(s). 2015
Received: 03/08/2014| Accepted: 28/10/2014| Published: 31/03/2015
Abstract

Methicillin-resistant S. aureus (MRSA) is one of the most important pathogens that cause a wide range of hospital and community-acquired infections worldwide. In the present study, the Multiplex PCR test was employed to detect the genes 16S rRNA (Staphylococcus genus specific), femA (encoding a factor essential for methicillin resistance), mecA (encoding high-level methicillin resistant) and lukS gene (encoding Panton-Valentine leukocidin [PVL]). The results showed that all isolates harbored the 16S rRNA. Fifty-six (56 %) of these were determined as methicillin-resistant S. aureus, while 21 (70 %) were methicillin-resistant coagulase-negative staphylococci. 9% of S. aureus isolates harbored the lukS gene. This multiplex PCR assay represents a simple, rapid, reliable approach for the detection of methicillin-resistant staphylococci, evaluate the frequency of virulence factor in community- associated  MRSA and discrimination of S. aureus from CoNS isolates.

Keywords

coagulase-negative Staphylococcus (CoNS), Panton-Valentine Leucocidin (PVL), Multiplex PCR, Methicillin

Article Metrics

Article View: 738

Share This Article

© The Author(s) 2015. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.