ISSN: 0973-7510
E-ISSN: 2581-690X
From spoiled cooked ham, six kinds of spoilage bacteria were isolated and three isolates were identified as Bacillus through matrix-assisted laser desorption/ionization time-off light mass spectrometry (MALDI-TOF MS). The results were further confirmed by 16S rRNA gene sequencing method at the species level. All these strains were found to be highly resistant to potassium sorbate and sodium nitrite, but greatly sensitive to both e-poly-L-lysine and nisin. Biopreservatives, especially, e-poly-L-lysine exhibited excellent inhibition on food spoilage microbial growth, significantly superior to a single chemical preservative, presenting a potential of improving food safety.
ε-poly-L-lysine, Nisin, Potassium sorbate, Sodium nitrite, 16S rRNA, Biotyper
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