ISSN: 0973-7510

E-ISSN: 2581-690X

Shakoor Ahmad Bhat and Malik Mehboob Willayat
Division of Veterinary Public Health, Faculty of Veterinary Sciences & Animal Husbandry
Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir,
Shuhama, Alusteng, Srinagar – 190 006, India.
J Pure Appl Microbiol. 2011;5(2):959-962
© The Author(s). 2011
Received: 15/02/2011 | Accepted: 20/03/2011| Published: 31/10/2011
Abstract

A total of 210 food samples (Fish-100, mutton-60, paneer-50) and 60 human clinical samples (blood-40, CSF-20) were screened for the isolation and identification of Listeria monocytogenes  using standard microbiological technique. Seven isolates of L. monocytogenes were recovered, and subjected to PCR assay for detection of virulence associated with hyl-A genes. The isolates under study produced strong amplification of the products at approximately 456 bp, which was in agreement with the expected size confirming the isolates as L. monocytogenes.

Keywords

Listeria monocytogenes, Amplification, Microbiological techniques

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