ISSN: 0973-7510
E-ISSN: 2581-690X
A total of 210 food samples (Fish-100, mutton-60, paneer-50) and 60 human clinical samples (blood-40, CSF-20) were screened for the isolation and identification of Listeria monocytogenes using standard microbiological technique. Seven isolates of L. monocytogenes were recovered, and subjected to PCR assay for detection of virulence associated with hyl-A genes. The isolates under study produced strong amplification of the products at approximately 456 bp, which was in agreement with the expected size confirming the isolates as L. monocytogenes.
Listeria monocytogenes, Amplification, Microbiological techniques
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