ISSN: 0973-7510

E-ISSN: 2581-690X

Naveen Kumar1, Yashpal Singh Malik2 , Satish Kumar3, Arvind Kumar3, Kuldeep Sharma4, Subhankar Sircar2 and Kuldeep Dhama5
1National Institute of High Security Animal Diseases (NIHSAD), Bhopal, India.
2Biological Standardization Division, Indian Veterinary Research Insitute (IVRI), Izatnagar, India.
3Division of Animal Biotechnology, Indian Veterinary Research Insitute (IVRI), Izatnagar, India.
4National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India.
5Division of Veterinary Immunology, Indian Veterinary Research Insitute (IVRI), Izatnagar, India.
J. Pure Appl. Microbiol., 2016, 10 (1): 447-450
© The Author(s). 2016
Received: 01/11/2015 | Accepted: 17/12/2015 | Published: 31/03/2016
Abstract

Bovine group A Rotavirus (RVA) is considered the major cause of diarrhea in neonatal calves. Use of peptides in diagnostics offers several advantages. In this study, we predicted regions within VP6 protein of bovine RVA showing high antigenic index using Bioinformatics tools and correspondingly synthesized linear peptides over Wang resin. Reactivity of individual peptides with anti-RVA polyclonal sera identified two immune-reactive epitopes, spanning 112-134aa and 125-149aa regions within the VP6 protein. Multiple antigenic peptides constructed from these regions could serve as a potential tool for the development of diagnostics and prophylactics.

Keywords

Diarrhea; Bovine Rotaviruses; Immuno-dominant epitopes; Peptides.

Article Metrics

Article View: 635

Share This Article

Journal Tools

© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.